Standardization of herbal drugs is the need of the hour as the use and practice of traditional herbal drugs has increased tremendously. The main objective of the present study is to standardize the flowers of Tupistra nutans as per pharmacopoeial testing protocol which includes powder microscopy, physico-chemical screening, TLC profile and chromatographic studies. Preliminary phytochemical screening ascertains presence of phytosterols in petroleum ether and triterpenoids in benzene extract. Alkaloid, phytosterols, glycosides & phenolics in chloroform extract. Carbohydrates, triterpenoids, glycosides, phenolics, tannins in methanol extract and proteins, glycosides, carbohydrates and flavonoids in aqueous extract of the plant flower extracts.TLC profiling of all plant extracts also give an idea about the presence of these phytochemicals. Rf (Retention factor) value of different phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals. Concentrating on results obtained in TLC, the column chromatographic studies were set. The fraction containing search here was flavonoids as, quercetin. Performing TLC of each individual fraction, same Rf value fractions were collected& concentrated during column chromatography.
