A new sensitive, simple, rapid and precise reversed phase high performance liquid chromatographic (HPLC) and a spectrophotometric method has been developed for estimation of tolterodine in tablet dosage form. Chromatography was performed by using the mobile phase, Potassium Phosphate pH 4.5 and acetonitrile mixed by a low pressure gradient program. Hypersil BDS C18 column was used. The wavelength detection was set at 205 nm. Linearity range for tolterodine was 10.0 – 60.0 μg/ mL. Linear calibration graphs values were found at 205 nm for tolterodine, The proposed method showed good linearity, precision and reproducibility and applied to the pharmaceutical dosage form containing the above mentioned drug without any interference by the excipients.
