A simple, reproducible and efficient reversed phase high performance liquid chromatography (RP-HPLC) method has been developed for estimation of a recently approved anti epileptic drug, Levetiracetam in raw material and its pharmaceutical formulations. Separation was done by using mobile phase consisting of diphasic sodium phosphate buffer and acetonitrile in a ratio of 80:20. The separations were carried out on an Inertsil C18 column (250x 4.6mm; 5μm) at a flow rate of 1.5 ml/min. The injection volume was 10 μl and the peaks were detected at 205nm. The linear dynamic response was found to be in the concentration range of 80 μg -130 μg/ml and coefficient of correlation was found to be 0.9994. The percentage recovery of levetiracetam was found to be 98.37%. The proposed method was found to be simple, fast, accurate, precise and reproducible and could be used for routine quality control analysis of levetiracetam in bulk and pharmaceutical dosage forms.
