The present work involves development and validation of RP-HPLC methods for simultaneous estimation of Cilnidipine and Metoprolol Succinate in their combined tablet dosage form. In HPLC method, Enable C18G (250 x 4.6 mm, 5 μm) column was used as stationary phase and Methanol: Water in the ratio 80: 20, (v/v) (pH adjusted to 3.5 with orthophosphoric acid) as mobile phase was used. The flow rate was 1 ml/min and both drugs were quantified at 231.0 nm. The retention time for Cilnidipine and Metoprolol Succinate was found to be 4.092 ± 0.12 min and 2.913 ± 0.18 respectively. The linearity range obtained for RP-HPLC method was 2 -10 μg/ml and 10 -50 μg/ml for Cilnidipine and Metoprolol Succinate respectively. The method was validated according to the guidelines of International Conference on Harmonization (ICH) and was successfully employed in the estimation of commercial formulations.
