Microcosmus exasperatus, a simple ascidian was analysed for the presence of water soluble vitamin B; fat soluble vitamins A, D, E and K by High Performance Liquid Chromatography and phenolic compounds, flavonoids by High Performance Thin Layer chromatography. Fat soluble vitamins D3, K and water soluble vitamin riboflavin and thiamine were noticed in high concentration. HPTLC studies revealed the presence of phenolic compounds such as Gallic acid, Ferulic acid and Caffeic acid. Rutin, Isoquercitrin and Quercetin were some of the flavonoids identified.
