The aim of current research was application of rhizobacteria as native biofertilizers and study of its effect on mint compounds change. Soil samples were collected from rhizosphere zone of Mentha L. and rhizobacteria were isolated on BHI agar, Nutrient agar and Soil extract agar media. The ability of isolated species to utilize nitrate, nitrite and phosphate solubility was assessed by spectrophotometer and Pikovskayas assay respectively and the best bacterial species were selected as biofertilizer. Pots of Mentha L. roots were inoculated by biofertilizer during 20 days at 75% humidity, 25°C temperature and 12 hours lighting and plant morphological was determined. Leaf and stem compounds change was evaluated by ethanolic extraction and GC/MS analysis. The best candidate bacterial species for use as biofertilizers were evaluated by 16S rRNA. 17 species with Bacillus genus as the most bacterial diversity were isolated and three strains were collected for further research. 11.7, 52.9 and 47% of strains had ability to use nitrite, nitrate and phosphorus solubility, respectively. The most size of leaf and internode distance was determined after treating the pots by strains 6 and 3 with average size 1.7 and 3.2cm, respectively. The results of synergetic effect of 3 strains were increased in leaf size and internodes distance, with average 1.8 and 4.4cm, respectively. Propene, Benzofuro benzopyran, Pentanoic acid, Decaborane, chloro Ã?Ë?Ã?Å?Heptan1-nitr Methoxycarbonyl and Hepten-1-ol were determined as the significant compounds in leaf plant sample which were inoculated with strains 3, 6, 10 and combination of three strains. Molecular analysis determined Bacillus subtilis, Bacillus endophyticus and Bacillus thuringiensis as strains 3, 6 and 10 respectively.
