A protocol of high efficient multiple shoot induction and plant regeneration from immature embryo and rhizome has been developed for Zingiber roseum – an endangered medicinal plant. The optimum (94%) of multiple shoots was obtained on woody medium supplemented with 3.21 μM NAA in combination with 17.68 μM BAP at 7.4 shoots per explants. In vitro shoots developed optimally (95.4%) rooted upon transferring onto the rooting medium with 2.68 μM NAA with 6.4 roots per shoot. The in vitro raised plantlets with well developed shoot and roots were successfully established in moistened sterile mixture of garden soil: sand: vermicompost in 1:1:1 ratio and grown in greenhouse for acclimatization at 23 ± 20C temperature and 18 h photoperiod with 30 μmol m-2 s-1at light intensity.
