The enzymes from microbial sources are more stable and obtained cheaply. Amylases are among the most important enzymes and are of great significance in present day industry. Starch degrading bacteria are most important for industries such as food, fermentation, textile and paper. Thus isolating and manipulating pure culture from various soil and waste materials has manifold importance for various biotechnology industries. In the present investigation bacterial strains were isolated from soil sample and growth pattern as well as optimum growth condition was determined. Characteristic feature of the strains indicates them as Bacillus subtilis, Pseudomonas florescens, Escherichia coli and Serratia marscens. The optimum temperature for production was 35-40 0C, whereas maximum growth was observed at 1% dextrose concentration but increases with increase in substrate concentration. The pH range was found to be 7 and incubation time 48hrs with 1ml as inoculum for optimum growth. Other optimum parameters include Yeast extract as Nitrogen source, Calcium chloride as chloride and Manganese sulphate as sulphate source for amylase production.
