The exorbitant costs of culture media have deprived the use of readymade culture media such as nutrient agar in schools and laboratories with less facility. Generally legume seeds are found to be a good protein source for nutritional purposes. This study was carried out to find the feasibility of using legume seeds as an alternative nutrient source to grow bacteria. Cowpea, green gram, black gram and soya meat (processed soya bean) were used in this study. The test organisms used were E. coli, Bacillus sp., Klebsiella sp., Staphyllococcus sp. and Pseudomonas sp. Staphyllococcus sp. grows well but slowly (332 – 356 CFU/0.1ml) and generally Klebsiella sp. (196 - 232 CFU/0.1 ml) grows least in all the protein formulations tested. All the tested bacteria grow least in green gram. In comparison with the performance on conventional nutrient agar media, the prepared protein formulations were found to be cheap and good alternative culture media for bacteriological studies.c
