Milk is a good source of many nutrients. However it could be a source of toxic substances like aflatoxin M1 (AFM1). The purpose of this study was to investigate of AFM1 contamination level in the marketed cow’s raw milk of Tabriz, Iran. A total of 10 marketed cow’s raw milk samples were collected randomly from Tabriz dairy stores between January to July 2009. Determination of AFM1 was based on ELISA (Enzyme Linked Immunosorbent Assay). Results show that in 5 samples (50%) the AFM1 concentrations were over the maximum tolerance limit (50ng/l) accepted by European Union and in 2 samples (20%) were over the maximum tolerance limit (100ng/l) accepted by the Iranian food standard. It can be concluded that AFM1 levels in the samples purchased in Tabriz city, appear to be a serious public health problem at the moment.