The prevalence and high susceptibility of aflatoxin contamination in agricultural commodities is alarming, posing great health concern to humans and animals. A total of twenty nine different types of Grains, Feeds and Feed ingredients were randomly obtained from two locations, Katsina (Sudan Savannah) and Zaria (Northern Guinea Savannah) metropolises. Raw samples were individually ground to pass through a 20-mesh screen and Total aflatoxins were extracted from the samples using 70% (v/v) methanol. Enzyme-linked immunosorbent Assay (ELISA) technique was used in analyzing aflatoxin concentration of the samples. Results shows that 79.3% of the entire sample were all positive with an aflatoxin level in the range of 0.1- >20 μg/kg. Amongst the grains, the highest concentration (>20 μg/kg) was found in white maize, while the least (0.1 μg/kg) was recorded in unmilled rice. For the feeds and feed ingredients, the highest concentration (>20 μg/kg) of aflatoxin was found in poultry feeds and maize chaff while the least concentration (0.85 μg/kg) was recorded in sorghum flour. Out of the positive samples 26.1% of them exceeded the SON and NAFDAC Aflatoxin permissible level. Though the aflatoxin concentration levels in samples were not disquieting, consistent consumption might result in long term accumulation of the toxin, causing an overt disease and an array of metabolic disturbances resulting in poor human and animal health
