European Journal of Zoological Research
Abstract
Author(s): M R. Pourelmi, M H. Palizdar, S. Shirali and A R. Barami
Aflatoxins are a highly toxic and carcinogenic mycotoxins which produced by Aspergillus species. This study was
done to determine the levels of aflatoxin contamination in feed, industrial and local eggs in west region of
Mazandaran in Iran. Sampling was done from all local and industrial units of egg production randomly and
simultaneously. 1400 industrial eggs from seven different zones with 5 replicate (in each replicate 40 eggs) and 700
traditional or local eggs from 7 points in 5 replications were collected. In addition 4 poultry feed samples with 5
replicate (Corn, Barley, Pletted diet and soybean meal) were collected. Aflatoxin B1 in samples was measured by
ELISA method. Aflatoxin B1 ELISA Test Kit of Europroxima that used in this research is based on competitive
enzyme immunoassay for the quantitative analysis of Aflatoxin B1 in cereals and feed. Tests and standards were
performed duplicated. Results showed aflatoxin contamination levels in local eggs were more than industrial
samples numerically (P > 0.05). Highest aflatoxin contamination was seen in Chalous local eggs (0.107 ng /ml) and
lowest contamination was seen in industrial egg from Sattari farm (0.050 ng /ml) respectively. These values were
lower than allowed limit (12 ng /ml). There was no significant difference between industrial eggs from different
farms of Mazandaran (P>0.05).
