Several hundred pesticides of different chemical moietiesare currently used for agricultural purposes all over the world. Because of their widespread use, they are detected in various environmental matrices, such as soil, water and air. Pesticide fate in the environment is affected by microbial activity. Microorganisms readily degrade some pesticides; others have proven to be recalcitrant. Recently the use of microbes for effective detoxification, degradation and removal of toxic compounds from contaminated soils has emerged as an efficient and cheap biotechnological approach to clean up polluted environments.This paper focused on the problem of soil contamination with pesticides, and present a review onthe potential applications of various biological agents for remediation of these types of soils, which have been polluted with continuous and higher doses of pesticides through process of biodegradation with special emphasis on advancements in organophosphorus pesticides biodegradation and clear understanding of its mechanism for the efficient onsite remediation of the contaminated environment.