Der Pharmacia Lettre
Abstract
Author(s): Keval L. Chaudhari, Rujuta D. Patel and Dilip G. MaheshwarI*
A new sensitive, simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Desvenlafaxine and Clonazepam in pharmaceutical dosage form. This method was based on UV spectrophotometric determination of two drugs, using absorbance correction method. It involves measurement of absorbances at two wavelengths 226 nm(�Ž�»max of Desvenlafaxine(DESV)) and 309 nm (�Ž�»max of Clonazepam (CLO)) in methanol for the simultaneous quantitative determination of Desvenlafaxine and Clonazepamin the binary mixture without previous separation. The linearity was observed in the concentration range 5-25 �Ž�¼g/ml for Desvenlafaxine and 5-25 �Ž�¼g/ml for Clonazepam. The accuracy and precision of the method was determined andvalidated statically. The method showed good reproducibility and recovery with % RSD less than 2. Method was found to be rapid, specific, precise and accurate, can be successfully applied for the routine analysis of Desvenlafaxine and Clonazepam in combined dosage form without any interference by the excipients. The method was validated according to ICH guidelines.
