An isocratic RP-HPLC Method for analysis of Rivastigmine in pharmaceutical dosage forms has been developed and validated. Best separation was achieved on a Thermo Hypersil C4 column (25 cm X 4.6 mm, 5 μm) using a mobile phase of 0.01 M ammonium acetate buffer adjusted to pH 4.0 with orthophosphoric acid and Acetonitrile (60:40, v/v) at a flow rate of 1.0 mL min-1. UV detection was performed at 220 nm. Atrovastatin was used as an internal standard. The retention time of Rivastigmine and Atrovastatin was 4.75 and 8.83 min, respectively. The method was validated for specificity, linearity, precision, accuracy, and limit of quantification, limit of detection, robustness, and solution stability. The proposed method was applied for the quantitative determination of Rivastigmine in commercial formulations.
