A simple, selective, rapid, precise and economical reverse phase high pressure liquid chromatographic method has been developed for the estimation of Oseltamivir from pharmaceutical formulation.The method was carried out on a Princeton SPHER C18 (25 cm x 4.6 mm i.d., 5 μ) column with a mobile phase consisting of acetonitrile: potassium Dihydrogen ortho phosphate (adjusted to pH 3.5 using orthophosphoric acid) (50:50 v/v) at a flow rate of 1.0 ml/min. Detection was carried out at 254 nm. Theophylline was used as an internal standard.The retention time of theophylline and oseltamivir was 5.56 and 9.61 min, respectively.The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability.The proposed method can be used for the estimation of oseltamivir in pharmaceutical dosage form.
