A simple, precise, accurate and selective reverse phase high pressure liquid chromatographic method has been developed and validated for the simultaneous estimation of Lopinavir and Ritonavir in bulk and pharmaceutical dosage form. The method was carried out on A HiQ sil C18 column (250 x 4.6mm id,5μm) in Isocratic mode with mobile phase consisting of methanol,acetonitrile and potassium dihydrogen phosphate (pH was adjusted to 5 by using Triethylamine) in the ratio of 70:10:20 at a flow rate 1.5 mL/min. The detection was carried out at 260 nm for both drugs. The retention times of Lopinavir and Ritonavir were found to be 3.73and 6.16 min respectively. The developed method was validated in terms of linearity, precision, accuracy, limit of detection/quantitation and robustness. The proposed method is easy to perform and successfully applied for the estimation of these drugs in bulk and tablet dosage form.
