Polluted pond is one of the specialized ecosystems. It is nutritionally rich, due to domestic sewage and industrial effluents as well as pesticides and hydrocarbons. The bacterial flora of Polluted water tends to be physiologically diverse due to the presence of rich nutrients. Microorganisms in such ecosystem utilize detritus matter and other available nutrients including PAH (Polycyclic aromatic hydrocarbon) break down compounds (Lillo and Rodriguez, 1990). Conventional microbiological techniques, based on the isolation of pure cultures and morphological, metabolic, biochemical, and genetic assays, have provided extensive information on the biodiversity of microbial communities in natural systems (Paramjit and Nitika, 2011). The bacterial flora in presence of rich nutrients tends to accumulate certain storage materials like volutin granules, lipids and polyhydroxyalkanoates (Du et al., 2004). Such a rich and diverse sewage ecosystem despite its potentials has not been adequately explored, for bacteria accumulating polyhydroxyalkanoates (PHA), and hence considered to be the potential environment for screening of bacteria accumulating PHA.
