The objective of this study was to evaluate the effects of seed pyridoxine-priming duration on germination and early seeding growth characteristics of two wheat genotypes included inbred lines of PBW-154 and PBW-343. The experiments were carried out in completely randomized design (CRD) with five seed priming treatments in three replications. The seed priming treatments included three pyridoxine-priming duration treatments consist of 6, 12 and 24 h were compare with the unsoaked seed control and a hydro-priming with distilled water for 12 h. The pyridoxine concentration of 200 mg 1-1 prepared in distilled water was used as pyridoxine-priming media. Seed pyridoxine-priming treatments improved seed germination and early seeding growth traits included germination percentage, coleoptiles and radical length, seeding dry matter accumulation, mean germination time (MGT), germination index (GI), vigor index (VI), and time to 50% germination (T50) of both genotypes. Seed pyridoxinepriming duration of 12 h produced maximum value for most of the germination and early seedling growth characteristics of wheat inbred lines of PBW-154 and PBW-343. These results have practical implications in the pre-sowing seed treatment with pyroxene solution could enhance the seed germination and early seedling growth characteristics of wheat plant.