A simple, precise, accurate and validated ultra performance liquid chromatography (UPLC) method has been developed for the estimation of Aprepitant and its impurities in capsule dosage form. The quantification was carried out using HSS C18 column, 100 x 2.1mm, 1.8μ and the mobile phase used was a mixture 0.01M Potassium di hydrogen phosphate and Acetonitrile (50:50 v/v) at a flow rate of 0.5 mL min-1. The detection wavelength was 210 nm at ambient temperature. The total run time was found to be 6 min. The method is linear with R2 values more than 0.999. The results obtained showed a good agreement with the stated content. Recovery values for Aprepitant and its impurities were 90% to 110%. The proposed method is reliable, rapid, precise, accurate and selective. The method was shown equivalency with the USP Method (Pending monograph, correspondence Number C89258); it can be used for regular analysis of Aprepitant in capsule dosage form.
