A rapid extractive spectrophotometric method is developed for the determination of copper (II) using o- Hydroxyacetophenoneisonicotinoylhydrazone (OHAPINH) as one of the sensitive analytical reagent. OHAPINH reacts with Cu(II) at pH 4.0 to form an yellowish brown colored complex, which can be extracted with dichloromethane (DCM). It is observed that the colour development is instantaneous and stable for more than 96 h. Beer’s law is obeyed in the range of 0.2-2.2 μg /mL of Cu(II), with a correlation coefficient 0.9998. The molar absorptivity (ε) and Sandell’s sensitivity of the complex are 4.8×104 L.mol-1.cm-1 and 0.0006 μg/mL, respectively. The metal to reagent ratio is found to be 1:2 by the method of Job’s continuous variation, mole ratio, Asmus and slope ratio methods. The optimized method has been successfully applied for the determination of copper (II) in the presence of diverse ions and also in biological, geological and pharmaceutical samples. It is also observed that the obtained results are in agreement with ICP-MS and AAS methods. The proposed method is selective, sensitive and reproducible.
