Weeds, being important sources of several lead molecules in compare to other plants and are less explored. There is a great scope for the discovery of newer anticancer molecules. Cancer being the most dreadful and resistant disease towards existing molecules, there is a current demand for the discovery of newer anticancer leads which are dual human topopoisons. Topoisomerases are ubiquitous enzymes that are essential during transcription and replication and dual topopoisonsare among the most effective and most commonly used anticancer and antibacterial drugs, Hence entire globe is in search for new dual human topopoisons. Hence the current research is focused on the discovery of new anticancer leads from the predominant weeds named Cytisus scoparius (Fabaceae), Cytisus scoparius (L.) Link (Fabaceae), Phytolacca dodecandra (Phytolaccaceae), Cnicus wallichi (Asteraceae) and Cestrum aurantiacum (Solanaceae). A novel compound (1E)-1-hydroxyprop-1-en-2-yl(2E)-3-(4-hydroxy- 3- methoxyphenyl)prop-2-enoate (A Ferulic acid derivative) was isolated first time from the aerial parts of Cytisus scoparius using flash chromatography technique. These isolated molecules proved to have significant in vitro wound healing activity against HDF cell lines at active concentrations of 26.5 μg/ml. The cell cycle analysis proved that these two compounds arrested cells at S phase & G2/M phase on HELA cell lines at concentration of 25 μg/ml. The topo-isomerase inhibition assays showed that these compounds are inhibiting topoisomerases I & II at concentration of 400 ug/ml. Hence these compounds can act as lead molecules for the discovery of potent dual topopoison I & II leads.
