A sensitive, simple, selective, reproducible and cost-effective spectrofluorimetric method for the determination of Amisulpride in bulk as well as commercial formulations. For the first time this method is based on reaction of Amisulpride with 1, 2 - Naphthoquinone -4- sulphonate (NQS) in sodium hydroxide to become a product of orange color. The spectrofluorimetric method involved in reduction of this product with potassium borohydrate, derivatized reaction product is obtained which is highly fluorescent product and shows maximum fluorescence intensity at λem = 370 nm after excitation at λex = 275 nm. The different experimental parameters affecting progress of the derivatization reaction were carefully studied and optimized. Under optimum experimental conditions the method has an excellent linear relationship with best correlation coefficient of 0.9999. The fluorescence intensity concentration plot was linear over the range of 0.2-1.2 μg/mL. The mean accuracy was found to be 99.50 % to 99.99 %. The intraday and interday precision was found to be 0.105-0.173 % and 0.116-0.133 % respectively. The limit of detection was found to be 0.0116 μg/mL and the limit of quantification was 0.0350 μg/mL. The interference effects on common excipients on the quantification of drug were investigated and no interference effects were observed. The results obtained were in good agreement with those obtained using a reported spectrophotometric method. Therefore this newly proposed spectrofluorimetric method is most convenient analytical technique and has been successfully feasible for determination of Amisulpride in bulk and pharmaceutical formulations in quality control as well as clinical laboratories.
