Tinospora cordifoliya (Gioe, Gudduchi or Amrita) is very well known in Ayurvedic system of medicine and found its use in various ailments. The present paper describes the isolation and structure elucidation of a new steroidal ester along with aliphatic alcohols and fatty acid esters from the alcoholic extract of Tinospora cordifolia stems. To carry out phytochemical investigation of dried stems of Tinospora cordifolia Miers. (Menispermaceae) for the isolation of a new steroidal ester. Column was eluted with different concentrations of petroleum ether: chloroform and their isolation and characterization was done with spectroscopic techniques. Results: Phytoconstituents characterized as stigmast-5-en-3β, 21-dio-3β-yl hexadecanoate (5) along with n-heptacosanol (1), n-octacosanol (2), n-nonacosanol (3), n-tetracontanol (4), n-triacontanyl palmitate (6) and n-hexacosanyl stearate (7). The structures of all these phytoconstituents have been established by means of chemical, spectral and chromatographic means.
