A facile method for synthesis of biologically stabilized silver nanoparticles was developed using the weed Parthenium hysterophorus L. The bio fabricated nanoparticles were characterized by UV-Vis spectrophotometry, XRay Diffraction and FTIR. The nanoparticles demonstrated potent antibacterial and antifungal activity against Staphylococcus aureus, Bacillus subtilis, Aspergillus niger and Escherichia coli. The silver nanoparticles synthesized thus demonstrated antagonistic activity against bacteria and fungi and hence the nanoparticles possess potential applications in medicine and pharmaceutical fields.