A new series of dihydro-1H-pyrazolo[3,4-d]pyrimidine and their derivatives were synthesized by using 4-(4- chlorophenyl)-3-methyl-1-phenyl-4,7-dihydro-1H-pyrazolo[3,4-d]pyrimidin-6-amine as a starting material. Twenty new heterocyclic containing a pyrazolo[3,4-d]pyrimidine ring were thus prepared in PEG-400 as a green reaction medium. The biological screening showed that most of these derivatives have good antimicrobial activities.The structures of new compounds were assignedon the basis of chemical and spectroscopic evidences.
