The aim of present work was to study comparative phenological events in diploid and tetraploid populations of Peganum harmala. In this paper an attempt has been made to record data regarding Sprouting, Flowering, Fruiting, and Senescence. This was done for a period of two years and it was carried out in two provinces (Kasmir and ladakh) of Jammu and Kashmir. Diploid and tetraploid individuals show divergent phenologies but the duration of different phases was similar. The different phases started approximately 1 month earlier in diploids than in tetraploids. Such phenological separation may arise because of genetically based differences between the two as a consequence of chromosome doubling or selection subsequent to the chromosome doubling event. Alternatively, phenological separations may arise if diploids and tetraploids occupy slightly different environments, which affect the timing and rate of growth.