Waste cooking oil (WCO) was purified from the impurities to improve the quality based on lipid profile measurement of biochemical serum parameters of experimental rats. The bio sorbent used are the shells powder of Anadara antiquata. The optimum condition of used oil purification was achieved with 15 g bio sorbent dose and 2 weeks of immersion. After purification process, the experimental rats were administered with the A. antiquata-treated (WCO) orally for 2 weeks. The measurement result of lipid profile indicate that administration of bio sorbent-treated used oil cannot reduce the level of malondialdehye (MDA), even there is an increase in the percentage of MDA as 18,67%, but it is lower if compared to WCO–treated rats as 29,79% in increasing of MDA levels. There are no significant changes in total cholesterol on A. antiquata treated wasted cooking oil, compared with WCO treated rats that there is increased of LDL levels as 11,47% compared to untreated rats. The increased of LDL levels in group A. antiquata treated WCO was 4,75%, which far lower than the increase in group WCO treated rats which is 43,12%. It can be concluded that A. antiquata has potential as ad adsorbent to improve the quality of lipid profile of waste cooking oil and oxidative stress
