This study was focused on the isolation and identification of some bacterial strains producing protease. For this purpose, a then 10 samples of protein rich soils from dairy factories, cattle’s feeding factories, and slaughterhouses, were collected from the region of Chief, located in Northwest Algeria, where the wastes are submitted to natural fermentation. The primary screening has been achieved on agar milk culture medium and the selection of the producing bacterial strains was based on the determination of the produced diameter of hydrolysis zone on the surface. The obtained results indicated the isolation 115 bacterial strains belonging to different genus. Furthermore, the 4selected protease producing strains were identified by the study of their biochemical characteristics, the amplification of the fragment ADN, coding the region of 16S ARN and their sequencing. The analyze of the amplified, sequenced DNA fragment, coding the region of 16S ARN fragments of the 4 selected protease producing strains by the using of RDP Project and BLAST), indicated their identification as Enterococcus faecium E745 and Enterococcus faecium PSB 5, respectively. Furthermore, the using of SDS-PAGE for protein profile indicated the presence protein bands around 35-37 KDa.
