According to World Health Organization more than 80% of the world's population relies on traditional medicine for their primary healthcare needs. The medicinal value of plants lies in some chemical substances that produce a definite physiological action on the human body. For the study these two plants was selected: Andrographispaniculata and Acacia Arabica respectively. Physiochemical as well as microscopic identification is important to authenticate the crude drug. For these different types of study design like Determination of Ash value, Determination of Total Ash, Acid insoluble ash value, Water soluble ash value, Sulphated ash, Determination of Extractive value, Extraction of Plant for Phytochemical screening, and Extraction by cold maceration. Under Quantitative Microscopy study: Determination of leaf constants, Stomatal number, Stomatal index, Vein termination number & vein islet number and Veinlet termination number was studied. Results of ash values for both plants showed higher in Andrographispaniculata5.50% than4.48%.in Acacia arabica. The results of extractive value of A. paniculata and A.arabica showed maximum extractive value in alcohol as compared to other polar and nonpolar solvents. Quantitative microscopical study of powdered leaves of Andrographispaniculata and Acacia arabica showed stomatal index 94 and 172 respectively. Similarly, stomatal index of A. paniculata was 50.8 and A. arabica was 25. Palisade ratio of A. paniculata in the range of 6-8 and A. arabica 5-6. Ash values are indicative to some extent of care taken in collection and preparation of drug for market and of foreign matter content of natural drug. The object of ash ing is to remove all traces of organic material interfering in an analysis of inorganic elements. Quantitative Microscopy is important tool in the identification of the plant and the differentiation of the closely related species. The mentioned results indicate that selected plant was genuine.
