The present work focuses essentially on the phytochemical screening and evaluate the antimicrobial activity of Both extracts from Satureja graeca L. ethyl acetate and n-butanol using a diffusion method for the most common pathogenic bacterial strains: E.coli ATCC 2592, Pseudomonas aerogenosa ATCC2783, Staphylococcus aureus ATCC 252923, Salmonella sp. Klebseilla pneumonia and one fungi Candida albicans. The results revealed the presence of some chemical groups such as volatile oils, flavonoids, sterols or triterpenes, tannins, carotenoids and saponins. Two extracts showed a good antibacterial activity against Staphylococcus aureus ATCC 252923.