Nanotechnology is currently exploited in the various fields of science and technology due to its unique effectiveness. Increased usage of nanotechnology principles, it is .necessary to study the non target effect. In the present study, the effect of chemically synthesized zinc oxide nanoparticles on the enzyme activity of amylase, and protease extracted from fungal isolate of Aspergillus niger, phytase from Hypocrea lixii SURT01 strain, cellulose and lipase from Tricoderma horzianum and Xylanase from Fusarium oxysporum under laboratory condition. All the tested enzymes were obtained from culture free supernatant and the crude enzyme thus obtained was evaluated for nanoparticles mediated enzyme inhibition by studying enzyme activity after the nanoparticles treatment. Notable changes in enzyme activity was not recorded in the enzyme activity of the tested enzymes except amylase which revealed increased concentration of nanoparticles inhibited enzyme activity. Distinct inhibition was not observed in the other tested enzymes.
