Research Article
Molecular Identification and Analysis of Chemical Constituents from Endophytic Mycoflora of Annona senegalensis Pers using Gas Chromatography-Mass Spectrometry and Fourier Transform Infrared Spectroscopy
Author(s): Ikechukwu A. Onah*, Dinebari P. Berebon, Thaddeus H. Gugu, Patrick N. Onyi, Franklin C. Kenechukwu, Emmanuel S. Onah, Emmanuel C. Ibezim and Anthony A. Attama
Standardized means of identification of endophytic organisms could become a necessary tool for the discovery and optimization of their vast bioactive metabolites. This study aimed to isolate, identified endophytic fungi from the Root (RT) and Leaves Blades (LB) of Annona senegalensis Pers using molecular techniques and analyzed the crude metabolites using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR). Endophytic fungi unknown (RT1), Botryosphaeria laricina (RT2), Didymella macrostoma (RT3) and Oxydothis ragae (LB1) associated with Annona senegalensis Pers were successfully isolated and identified. Based on the 18S rRNA gene sequences, four endophytic fungi, RT1, RT2, RT3 and LB1 were assigned GenBank accession numbers MW672177, MW672178, MW672179 and MW672180 inclusive under submission code SUB7061858. The result showed four endophytic.. Read More»
DOI:
10.4172/0976-1233.001
