Commentary
Utilizing a 3D human corneal epithelial tissue model to study oxidative stress in corneal injuries of various origin
Author(s): Elena William*
The current study’s goal was to promote ocular treatments by using a three-dimensional (3D) corneal epithelial tissue model to examine dry eye disease and oxidative stress-related corneal epithelial damage. Normal human corneal epithelial cells were grown in air-liquid interface cultures to create 3D corneal epithelial tissues suitable for physiologically relevant exposure to environmental variables. By subjecting the tissues to non-toxic amounts of ultraviolet (UV), hydrogen peroxide, the vesicant nitrogen mustard, or desiccating circumstances, oxidative stress was created. These stimuli induced morphological, cellular, and molecular alterations related to dry eye illness. Barrier function, tissue viability, reactive oxygen species (ROS) accumulation, lipid peroxidation, cytokine release, histology, and gene expression were all assessed as part of the evaluation of the specific.. Read More»
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