Review Article
The Review on Simultaneous Estimation and Validation of Tetracycline Derivatives and their Metabolites Using HPLC
Author(s): Abhishek Kumar*, Prawal Kumar Gupta, Pratibha Rana, Ashu Goel, Afsha Khan and Saurabh Singh
A excessive overall performance liquid chromatography technique utilising ultraviolet detection turned into advanced and established to decide tetracyclines residues in Anhydrotetracycline (ATC), Epianhydrotetracycline (EATC), Epitetracycline (ETC), 4-Epi-Chlortetracycline (ECTC), 4-Epianydrochlotetracycline (EACTC), 4-Epioxychlortetracycline (EOTC) samples. The separation of 3 tetracyclines residues; Tetracycline (TC), Chlortetracycline (CTC), Oxytetracycline (OTC) turned into performed on a reverse-segment C eight column with a gradient elution. A cellular segment device consisted of a combination of methanol, acetonitrile and 0.03M oxalic acid.
UV detector set 351 nm with 25 minute evaluation time. The extraction with citrate buffer pH four turned into carried out and observed through SPE cartridge easy up step. Calibration curves for Tetracycline (TC), Chlortetracycline .. Read More»
DOI:
10.37532/dpl.2022.14.15
