Cell culture is an essential technique in biomedical research, enabling the study of cellular processes in a controlled environment. Central to successful cell culture is the formulation of appropriate media that support cell viability, growth, and differentiation. The composition of cell culture media is essential because it provides essential nutrients, growth factors, and signaling molecules required cells to thrive and reproduce. Enhancing cell viability through optimized media formulations is an ongoing area of research, particularly as the demand for high-quality cell culture grows in drug development, regenerative medicine, and tissue engineering.