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Quantitative Estimation of Quercetin in Mimusops elengi L. (Bakul) Leaves by HPTLC | Abstract
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Abstract

Quantitative Estimation of Quercetin in Mimusops elengi L. (Bakul) Leaves by HPTLC

Author(s): Sakshi Sehgal, Vineet Gupta, Rajiv Gupta, Shubhini A. Saraf

Mimusops elengi L. is a medicinally valuable herb in the Ayurvedic and traditional systems of medicine. Various activities have been reported in almost all parts of Mimusops elengi, some of which includes diuretic activity, antidiabetic, antibacterial etc. Quercetin, one of the most important flavanoid is active against various cardio vascular diseases, cancer, tuberculosis, neurological diseases, cataract etc. In the present study High Performance Thin Layer Chromatography has been developed for detection and quantification of quercetin in Mimusops elengi leaves. Increasing serial dilutions of reference standard quercetin (200 to 1000 μg/mL) were scanned at 366 nm to detect and quantify the concentrations of quercetin in the test sample.The estimated values obtained from the same was 19.191mg/gm quercetin in powdered leaf sample.The method provided a rapid and easy approach for detection and the quantitation of the bio-marker quercetin. The authors also aim to validate the present method in terms of ruggedness and accuracy and undertake the isolation of quercetin from the said plant.