Evaluation of antinociceptive and anti-in?ammatory properties of Desmodium gangeticum (L.) in experimental animal models

By Manoj Kumar Sagar, Aadesh Upadhyay, Kalpana and Kumud Upadhyaya

ABSTRACT
The present study was carried out to investigate the anti-inflammatory and antinociceptive activity of the ethanolic extract of Desmodium gangeticum leaves. We evaluate the ethanolic extract against thermal (Eddy’s hot-plate & Tail flick test) and chemical (Acetic acid & Formalin) induced nociceptive response as well as anti-inflammatory activity against Carageenan induced paw oedema. The oral administration of DG extract (50, 100, 200mg/kg), positive control morphine (5mg/kg i.p.) and aspirin (300mg/kg o.p.) inhibited acetic acid-induced writhing by 25.92%, 55.12%, 68.13%, 85.61% and 72.19% (P<0.05), respectively. The highest dose of the DG extract increases the latency period by 37.65% in Eddy’s hot-plate and 28.26% (P < 0.05) in tail fick test. In formalin induced nociceptive pain, 200mg/kg dose of extract inhibits 29.67% (P < 0.05) of neurogenic pain and in late inflammatory phase as compared to diclofenac (45.31%) & indomethacin (44.39%), DG extract shows 30.87%(100mg/kg), 42.78%(200mg/kg) inhibition. In carageenan induced paw oedema model, DG extract shows 15.68% (50mg/kg), 24.5% (100mg/kg) and 45.09% (200mg/kg) inhibition after 6h, where indomethacin shows 51.96%. After 24h, 200mg/kg extract dose and indomethacin were equipotent by inhibiting 43.86 %, 46.57%, respectively.  These results indicate the presence of anti-inflammatory and antinociceptive principles in the ethenolic extract of Desmodium gangeticum, and reinforce the plant’s potential therapeutic use against pain and inflammatory diseases.

Key words: Antinociceptive activity; Anti-inflammatory activity; Desmodium gangeticum; oedema; indomethacin.
Abbreviations: DGE:  Ethanolic extract of Desmodium gangeticum.

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