Study on adsorption trends of bovine serum albumin onto metallic surface

By Awe, F. E., Okunola, O. J., Zubairu, S. M. J., Omeiza, F .S. and Lori, J. A.

The hypothesis that the adsorptive affinity of macromolecules to titanium, Ti, is enhanced with the presence of Ca2+ was tested, by dissolving the adsorbate in Hanks’ solution. The study investigated the adsorption ability of Bovine Serum Albumin, BSA (dissolved in Hanks’ solution) onto Ti powder. The amount of BSA adsorbed increases with contact time. Saturation of the binding sites of Ti surfaces by BSA took place at 45minutes contact time. The amount of BSA adsorbed onto Ti at 30minutes contact time for all the experimental groups was 1.5times higher than that at 15minutes. The adsorption isotherms of BSA onto Ti exhibited the Langmuir type, with maximum of 0.138mg per gram of Ti powder. The BSA-Ti association constant was 4.62ml/mg. Pretreatment of Ti with calcium, or magnesium alone, resulted in augmented adsorption of BSA onto Ti. No increase in adsorption was observed following pretreatment of Ti with potassium. These results point to the involvement of electrostatic interaction in adsorption of BSA onto Ti. Study of the adsorptive strength indicated strong adsorptive ability of BSA onto Ti, at the eighth rinse no more BSA molecules was adsorbed onto Ti.

Key words:  Bovine Serum, Titanium, Adsorption, Characeristics

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