Validated UV spectroscopic method for estimation of Salbutamol from tablet formulations

By Arun K. Mishra, Manoj Kumar, Amrita Mishra, Anurag Verma, Pronobesh Chattopadhyay


Abstract

A simple, sensitive and specific UV spectrophotometric method was developed for the estimation of Salbutamol in tablet dosage form. The optimum conditions for the analysis of the drug were established. The wavelength maxima (?max) for Salbutamol were found to be 276 nm. The linearity for this method was found to be in the range of 10-120µg/ml. The method showed high sensitivity with reproducibility in results. The lower limit of detection and the limit of quantification were found to be 4.234and 12.702 respectively. The calibration curve was drawn by plotting graph between absorbance and concentration. Coefficient of correlation was higher than 0.99. The regression of the curve was Y = 0.002x + 0.0821. Precision of the method was found to be 1.625 ± 0.324 against the label claim of 4mg. The percentage recovery was found to be 98.56±0.238. The sample solution was stable up to 12 hours. The proposed method may be suitably applied for the analysis of Salbutamol in tablet pharmaceutical formulation for routine analysis.

Key words: Salbutamol, UV Spectroscopy, Tablet dosage form.

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