Effect of bacterial and viral vaccines on bovine - A spectral analysis

By A.Rajalakshmi, S.Gunasekaran and T.S.Renuga Devi


Livestock are to be regularly vaccinated to protect them from many infectious and contagious diseases. Anthrax is a disease of mammals, including human, is caused by a spore-forming bacterium called Bacillus anthracis. Anthrax Spore Vaccine (ASV) is a glycerinated suspension of live spores of uncapsulated avirluent strain of Bacillus anthracis. ASV can be used to protect all species of animals viz, cattle, sheep, goat, horse, ass, elephant, pigs and camel. Foot and Mouth Disease (FMD) is an acute, highly contagious picornavirus infection of cloven hoofed animal. The virus exists as seven serotypes: A, O, C, Asia1, SAT I, SAT II, and SAT III. Cattle are mainly infected by inhalation, often from pigs, which excrete large amounts of virus by respiratory aerosols and are considered highly important in disease spread. FMD monovalent vaccine is a liquid preparation containing any one type (O, A, C, ASIA-I) of FMD virus inactivated with formalin and adsorbed on buffered aluminium hydroxide gel.  Two groups of five animals were vaccinated with anthrax spore vaccine (bacterial) and foot and mouth disease vaccine (viral). Pre and post vaccinated sera samples of cattle were collected and tested using Fourier Transform Infra Red (FTIR) spectrometer. Spectroscopic method of blood analysis is an alternate technique to the clinical method since they require fewer samples and provide more information. The variation in peaks was due to the change in protein and lipid levels in the animals due to introduce of antigens. The present work can be extended and compared with the other immunology tests in future.

Key Words:  Fourier Transform Infra Red (FTIR) spectrometer, anthrax spore vaccine, foot and mouth disease vaccine, uncapsulated , avirulent, antigen.


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