Cytotoxic Effects of Tabebuia Rosea Oils (Leaf and Stem Bark)

By G.K. Oloyede, I.A. Oladosu, A. F. Shodia and O.O. Oloyade


The leaf and stembark of Tabebuia Rosea were separately collected, dried and grounded. The powdered samples were subjected to distillation using a hydro-distiller (all-glass clevenger apparatus), to extract the essential oil present in the plant samples. GC and GC/MS analysis were carried out on the essential oils and were found to contain a total of five and six compounds in the leaf and stembark respectively. The leaf contained o-xylene (2.13%), 2,4-dimethylhexane (1.03%), methyl cyclohexane (53.13%), methyl benzene (12.75%), 3-Pentene-2-one(0.11%) representing 69.15% of the total essential oil while the stem bark contained  n-amyl ketone (46.69%), methyl cyclohexane (24.07%), methyl benzene (13.88%), a –carene (0.46%), ß-carene (0.46%),and  ?-carene (0.46%) representing 85.62% of the total essential oil. The toxicity of these oils was shown by brine shrimp test. The LC50 value (µg/ml) of 1.701 with upper confidence limit and lower confidence limit of 2.137 and 0.4678 respectively for both the leaf and stem bark indicated that the oils were highly toxic.

Key words: Tabebuia rosea, cytotoxicity, hydrodistillation, Gas Chromatography/ Mass Spectroscopy.


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