By C. D. Shajiselvin and A. Kottai Muthu
The study was designed to examine the invitro free radical scavenging activity of various extracts of whole plant of Borreria hispida. The antioxidant activity was evaluated by Hydroxyl radical scavenging activity, Nitric oxide radical scavenging activity with reference standard Ascorbate and total phenol content respectively. An IC50 value was found that methanolic extract of Borreria hispida is more effective in hydroxyl radical scavenging activity than that of d petroleum ether and ethyl acetate extracts. The methanolic extract of Borreria hispida was found to more effective in the nitric oxide radical scavenging activity. The IC50 values of the methanolic extract of Borreria hispida and ascorbate were found to be 190µg/ml and 410µg/ml respectively. But when compare to the all the three extracts with ascorbate (standard), the methanolic extract of the Borreria hispida showed the better result. In addition, the methanolic extract of Borreria hispida was found to contain a noticeable amount of total phenols, which play a major role in controlling antioxidants. Moreover, the results were observed in a concentration dependent manner. So, the invitro studies clearly showed that the methanolic extract of Borreria hispida has a significant antioxidant activity. These invitro assays indicate that this plant extracts is a better source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.
Key Words : Whole plant of Borreria hispida, invitro antioxidant, hydroxyl radical scavenging activity, nitric oxide radical activity, total phenol.
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