A validated RP-HPLC method for estimation of Rivastigmine in pharmaceutical formulations
By S. Alexandar, Rohini Diwedi, T. Ashok and M. J. N. Chandrasekhar
ABSTRACT
An isocratic RP-HPLC Method for analysis of Rivastigmine in pharmaceutical dosage forms has
been developed and validated. Best separation was achieved on a Thermo Hypersil C4 column
(25 cm X 4.6 mm, 5 μm) using a mobile phase of 0.01 M ammonium acetate buffer adjusted to
pH 4.0 with orthophosphoric acid and Acetonitrile (60:40, v/v) at a flow rate of 1.0 mL min-1.
UV detection was performed at 220 nm. Atrovastatin was used as an internal standard. The
retention time of Rivastigmine and Atrovastatin was 4.75 and 8.83 min, respectively. The method
was validated for specificity, linearity, precision, accuracy, and limit of quantification, limit of
detection, robustness, and solution stability. The proposed method was applied for the
quantitative determination of Rivastigmine in commercial formulations.
Keywords: Rivastigmine, RP-HPLC, Method development.
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