Evaluation of In-vitro antioxidant methods of cassia auriculata 

By Gaurav M. Doshi , Mukesh D. Shahare, Gayatri V. Aggarwal, Preeja G. Pillai and Sandhya K. Desai 

ABSTRACT

Antioxidant studies were carried out for methanolic extract of Cassia auriculata seeds for proving its utility in inflammation and healing mechanism. The methanolic extract was screened for antioxidant activity by nitric oxide radical scavenging, lipid peroxidation inhibition and DPPH methods at different concentrations. Throughout the studies seeds extract showed marked antioxidant activity. The antioxidant activity of the seeds extract may be due to stabilization of plasma membrane, thereby lowering the elevated levels of serum lysosomal enzymes. The antioxidant activity was found to be concentration dependent and may be attributed to the presence of high flavanoids and bioflavonoids content in the seeds of Cassia auriculata.

Keywords: Free radicals, 1, 1- diphenyl-2-picrylhydrazyl, Spectrophotometer , In-vitro, nitric oxide. 

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