A simple and sensitive spectrophotometric method for estimation of
diethylene triamine penta acetic acid (DTPA) in topical gel formulations
By Sunil Kamboj, Deepak Sharma, Anroop B Nair, Suman Bala,Rakesh Kumar Sharma,
Vinod Kumar, Javed Ali, Pramod K and S. H. Ansari
A simple, sensitive, economical and reproducible UV-Spectrophotometric method was developed
for the estimation of DTPA in topical gel formulations. The method is based on NaFeDTPA
complex formation by the reaction of DTPA with ferric chloride in 0.1N HCl. Optimum
conditions for the reaction were investigated and absorbance was read at λmax at 272 nm. Bears
law was obeyed in the range of 5μg/mL-50μg/mL with correlation of 0.9998. The detection and
quantitation limits were found to be 0.8701 and 2.6366 μg/mL respectively. The proposed
method was successfully applied for the determination of DTPA in topical gel formulations.
Accuracy was examined through recovery studies. The results show that the procedure is
accurate, precise and reproducible (relative standard deviation < 1 %), while being simple, cost
effective and less time consuming, which proves the suitability of the proposed method for the
estimation of DTPA in topical gel formulations.
Keywords: DTPA, U.V Spectrophotometric method, Topical gel.
Click here to download the complete article in PDF Format