In vitro antimicrobial activity of Clerodendrum viscosum (Vent)
By Richard lobo, K.S Chandrshakar, Jaykumar. B, Mamatha Ballal
Clerodendrum viscosum a common plant belonging to the family verbenaceae , grows as a weed on roadside and waste lands. The various extracts of the leaves of C.viscosum were prepared by successive solvent extraction with increasing polarity order using soxhelt apparatus. The various extracts prepared were subjected to antimicrobial screening for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Shigella and ,Vibrio cholerae at a concentration of 20, 60, 100mg/ml in DMSO. Punch well method is used to acess the activity, the results obtained showed that the petroleum ether fraction exhibited a significant activity against Staphylococcus aureus, chloroform extract showed activity against Staphylococcus aureus .Pseudomonas aeruginosa, Escherichia coli, Shigella, Vibrio cholera, acetone extract showed significant activity against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae,Shigella,Vibrio cholerae and alcoholic extract exhibited the activity against Pseudomonas aeruginosa Klebsiella pneumoniea, Shigella, Vibrio cholerae. Benzene and aqueous extract did not show any activity against any of the pathogens used in the test.
Key words: Clerodendrum viscosum (Vent), Mueller Hinton Agar, Successive extraction, Clerodin, Antimicrobial activity.
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