Development and Validation of LC Method for Simultaneous Determination of Piroxicam and Paracetamol in New Pharmaceutical Formulation 

By A. B. Devkhile and K. A. Shaikh 

ABSTRACT

In this perspective, we present a reverse phase high performance liquid chromatographic method for simultaneous determination of piroxicam and paracetamol in commercial pharmaceutical dosage forms. The resulting contents of tablets were baseline resolved on a reverse phase Zorbax SB C18, (250 x 4.6 mm, 5 mm) analytical column. Mobile phase contains methanol, acetonitrile and 50 mM sodium dihydrogen orthophosphate (27:16:57 v/v) at a flow rate of 1 mL/min. UV detector was set at a wavelength of 254 nm. The resolution between paracetamol and piroxicam was less than five. Developed method was extensively proved to be robust for the titled drugs. This method was shown to be linear, correlation coefficient of paracetamol and piroxicam was 0.9986 and 0.9990 respectively. The percent recovery for paracetamol and piroxicam ranged between 100.5-101.3 and 100.6-100.8 respectively. The percent relative standard deviation for six replicates was less than 2 .The limit of detection and limit of quantification for paracetamol was 140 ng / mL and 400 ng / mL for piroxicam 29 ng / mL and 70 ng / mL . Sample concentrations were measured on weight basis to avoid internal standard. Proposed method is suitable for quantitative determination of the titled drugs in their commercial samples of tablet formulation with respect to assay. 

Keywords : Piroxicam, Paracetamol, Liquid chromatography, Pharmaceutical formulation, Method validation

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