Validated spectrophotometric methods for determination of Frovatriptan Succinate Monohydrate in pharmaceutical dosage forms

By Sasmita Kumari Acharjya, Pinakini Panda, Priyambada Mallick and M. Mathrusri Annapurna

 

ABSTRACT


UV, first derivative, second derivative and AUC-spectrophotometric methods for the determination of Frovatriptan Succinate Monohydrate (FSM) in pharmaceutical formulations have been developed. For the first method, UV-spectrophotometry, standard solutions were measured at 279 nm. The linearity ranges were found to be 1–80 µgml-1 in 0.1N HCl and the regression equation was A=1.8866×10-2C-2.3397×10-3 (r=0.9992). For the second method, first derivative spectrophotometry, the response (dA/d?) of standard solutions was measured at 292 nm. Calibration curve was constructed by plotting dA/d? values against concentrations, 2.5–80 µgml-1 of FSM standards in 0.1N HCl. Regression equation of linear calibration graph was calculated as D1= -6.36×10-4C-1.73×10-4(r=0.9997). For the third method, second derivative spectrophotometry, the response (d2A/d?2) of standard solutions was measured at 281 nm. Calibration curve was constructed by plotting d2A/d?2 values against concentrations, 10–80 µg ml-1 of FSM standards in 0.1N HCl. Regression equation of linear calibration graph was calculated as D2= -9.7×10-5C – 6.1×10-5 (r=0.9995). The fourth method was based on calculation of Area under Curve (AUC) for analysis of FSM in the wavelength range of 274–284 nm. Calibration curve was constructed by plotting AUC values against concentrations, 1–80 µg ml-1of FSM standards in 0.1N HCl. Regression equation of linear calibration graph was calculated as AUC=0.1855C-0.0246 (r=0.9992). The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of FSM in pharmaceutical formulations.

Key words: Frovatriptan Succinate Monohydrate; UV-spectrophotometry; Derivative-spectrophotometry; AUC- spectrophotometry.

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