In vitro evaluation of polymeric beads of riboflavin formulated at different cross-linking time

By Manjul Pratap Singh, Gulzar Alam, Roshan Patel, Upendra Kumar and Anita Singh


Sodium alginate beads were evaluated as sustained release vehicles for the delivery of Riboflavin. It has been shown that the structure of the cross-linked calcium alginate network is of primary importance in the retention and/or release of the Riboflavin. The Riboflavin bead formulations were prepared by dispersing Riboflavin together with a mixture of   sodium alginate and plasticizer solution and then dripping the dispersion into a solution of 5% (w/v) calcium chloride for different time of cross linking. Prepared beads were evaluated for Particle size, Flow property, Drug entrapment efficiency and In-vitro drug release. The formulations showed the particle size and angle of repose within acceptable range having good flow property but the beads formed by high cross linking time demonstrated nearly same particle size and showed poor flow property as compared with other formulations.  Drug entrapment efficacy of all formulations was in the range of 55.76 - 74.21% and entrapment efficacy of beads decreases with increase the cross linking time. Riboflavin In-vitro release from beads was studied. The Beads prepared by maximum 15 minutes cross linking time shows 77% drug release. The values of coefficient correlation were calculated and drug release was found to follow Higuchi matrix order release.

Key words:  Sodium alginate, riboflavin, angle of repose and cross linking time.

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